Pro-apoptotic effect of the landrace Bangla Mahoba of piper betle on Leishmania donovani may be due to the high content of eugenol

Misra, P. ; Kumar, A. ; Khare, P. ; Gupta, S. ; Kumar, N. ; Dube, A. (2009) Pro-apoptotic effect of the landrace Bangla Mahoba of piper betle on Leishmania donovani may be due to the high content of eugenol Journal of Medical Microbiology, 58 (8). pp. 1058-1066. ISSN 0022-2615

Full text not available from this repository.

Official URL:

Related URL:


In the absence of effective and safe treatment for visceral leishmaniasis or Kala-azar – a devastating parasitic disease caused by Leishmania donovani – the search for anti-leishmanial agents from natural resources in common use is imperative. Recently, the comparative in vitro anti-leishmanial activity of methanolic extracts from two landraces of Piper betle – P. betle landrace Bangla Mahoba (PB-BM) and P. betle landrace Kapoori Vellaikodi (PB-KV) – has been reported. Here, the putative pathway responsible for death induced by the effective extract of PB-BM methanolic extract in promastigotes, as well as the intracellular amastigote form of L. donovani, was assessed using various biochemical approaches. It was found that PB-BM was capable of selectively inhibiting both stages of Leishmania parasites by accelerating apoptotic events by generation of reactive oxygen species targeting the mitochondria without any cytotoxicity towards macrophages. The study was extended to determine the presence or absence of activity of the methanolic extract of PB-BM and PB-KV on the basis of differences in essential oil composition present in the extract assessed by GC and MS. The essential oil from PB-BM was found to be rich in eugenol compared with that from PB-KV. The anti-leishmanial efficacy of PB-BM methanolic extract mediated through apoptosis is probably due to the higher content of eugenol in the active landrace. This observation emphasizes the need to extend studies related to traditional medicines from bioactive plants below the species level to the gender/landrace level for better efficacy and reproducibility.

Item Type:Article
Source:Copyright of this article belongs to Society for General Microbiology.
Keywords:VL; Visceral Leishmaniasis; Pb-Bm; Piper Betle Landrace Bangla Mahoba; Fiu; Fluorescence Intensity Unit; Pb-Bmm; Pb-Bm Methanolic Extract; Fitc; Fluorescein Isothiocyanate; Pb-Bme; Pb-Bm Essential Oil; Pb-Kv; Piper Betle Landrace Kapoori Vellaikodi; Pb-Kve; Pb-Kv Essential Oil; Pb-Kvm; Pb-Kv Methanolic Extract; Ki; Kovats Index; H2dcfda; 2′,7′-Dichlorodihydrofluorescein Diacetate; Ros; Reactive Oxygen Species; Ic50; Concentration Inhibiting Cell Growth By 50 %; Pi; Propidium Iodide; Tunel; Terminal Deoxyribonucleotidyl Transferase-Mediated Dutp Nick End Labelling; Gfp; Green Fluorescent Protein; Mfi; Mean Fluorescence Intensity
ID Code:99158
Deposited On:09 Nov 2015 04:20
Last Modified:09 Nov 2015 04:20

Repository Staff Only: item control page