Systematic characterization of the conformation and dynamics of budding yeast chromosome XII

Albert, B. ; Mathon, J. ; Shukla, A. ; Saad, H. ; Normand, C. ; Leger-Silvestre, I. ; Villa, D. ; Kamgoue, A. ; Mozziconacci, J. ; Wong, H. ; Zimmer, C. ; Bhargava, P. ; et al., (2013) Systematic characterization of the conformation and dynamics of budding yeast chromosome XII The Journal of Cell Biology, 202 (2). pp. 201-210. ISSN 0021-9525

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Official URL: http://jcb.rupress.org/content/202/2/201

Related URL: http://dx.doi.org/10.1083/jcb.201208186

Abstract

Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ~100 kb along the chromosome, and investigated their motion over broad time scales (0.2-400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion.

Item Type:Article
Source:Copyright of this article belongs to Rockefeller University Press.
ID Code:98046
Deposited On:29 Jan 2014 11:01
Last Modified:29 Jan 2014 11:01

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