Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica

Gupta, Abhishek Kumar ; Panigrahi, Sunil Kumar ; Bhattacharya, Alok ; Bhattacharya, Sudha (2012) Self-circularizing 5′-ETS RNAs accumulate along with unprocessed pre ribosomal RNAs in growth-stressed Entamoeba histolytica Scientific Reports, 2 . Article ID 303, 8 pages. ISSN 2045-2322

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Official URL: http://www.nature.com/srep/2012/120306/srep00303/f...

Related URL: http://dx.doi.org/10.1038/srep00303

Abstract

The primary transcript of rRNA genes is a large pre rRNA which is precisely processed to release the mature rRNAs. The 5′-external transcribed spacer (ETS) of rRNA genes contains important sites for pre rRNA processing. Once the processing is accomplished the ETS is rapidly degraded. We show that in growth-stressed cells of the human parasitic protist Entamoeba histolytica the A'-A0 sub-fragment of the 5′-ETS accumulates to high levels as a family of RNA molecules of size 666 to 912 nt. These etsRNAs are circular in vivo and can spontaneously self-circularize in vitro. The accumulation of etsRNAs is accompanied by accumulation of unprocessed pre rRNA, indicating a possible role of etsRNAs in inhibition of processing during growth stress. Our data shows for the first time that processed etsRNA is not a mere by-product destined for degradation but is stabilized by circularization and could play a regulatory role as noncoding RNA.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
ID Code:98043
Deposited On:29 Jan 2014 11:30
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