Abrogation of DTH response and mitogenic lectin- and alloantigen-induced activation of lymphocytes by calcium inhibitors TMB-8 and BAPTA-AM

Saxena, Hari Mohan ; Dikshit, Madhu (2005) Abrogation of DTH response and mitogenic lectin- and alloantigen-induced activation of lymphocytes by calcium inhibitors TMB-8 and BAPTA-AM Immunology Letters, 101 (1). pp. 60-64. ISSN 0165-2478

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01652...

Related URL: http://dx.doi.org/10.1016/j.imlet.2005.04.011

Abstract

In vitro treatment of mouse lymphocytes with an intracellular calcium chelator BAPTA-AM significantly decreased lectin Concanavalin-A (Con-A)-induced and mixed lymphocyte reaction (MLR) mediated, alloantigen-induced lymphocyte activation as indicated by decreased percentage of lymphoblasts among the BAPTA-treated lymphocytes. In vivo treatment of mice with intracellular Ca2+ antagonist TMB-8 was found to substantially impair delayed type hypersensitivity (DTH; cell mediated immune) response, as indicated by decreased footpad swelling on tuberculin challenge of mice sensitized with BCG, after a single treatment with a low dose of 0.01 mg of TMB-8 per mouse. Interestingly, a second injection of a higher dose of TMB-8 (0.1 mg per mouse) resulted in very significant (p = 0.001) abrogation of DTH as indicated by complete absence of swelling of foot pad after PPD challenge in BCG-primed treated mice. All mice in this group showed fully impaired DTH response. Lymphocytes of allosensitized mice gave a significantly higher (p < 0.05) MLR response than the naive mice. However, a single treatment of allosensitized mice with 0.1 mg TMB-8 resulted into a lower MLR response, comparable in magnitude to that of untreated naive mice.

Item Type:Article
Source:Copyright of this article belongs to European Federation of Immunological Societies.
Keywords:Calcium Inhibitors; DTH; Alloactivation; TMB-8; BAPTA-AM
ID Code:9691
Deposited On:30 Oct 2010 11:23
Last Modified:09 Feb 2011 04:42

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