In vitro expression of belladonna mottle virus genome

Jacob, A. N. ; Savithri, H. S. (1991) In vitro expression of belladonna mottle virus genome Indian Journal of Biochemistry and Biophysics, 28 (5-6). pp. 456-460. ISSN 0301-1208

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In vitro translation of belladonna mottle virus BDMV(I) genomic RNA in a rabbit reticulocyte lysate system produced proteins of Mr 210,000, 150,000 and 78,000 which form the non-structural proteins. The coat protein, on the other hand, was expressed from a subgenomic RNA which was found to be encapsidated in the empty capsids forming the top component viral particles. The implications of subgenomic RNA encapsidation in viral replication and assembly are discussed.

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ID Code:96599
Deposited On:28 Dec 2012 11:39
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