Kinetic studies of the folding of heterodimeric monellin: evidence for switching between alternative parallel pathways

Aghera, Nilesh ; Udgaonkar, Jayant B. (2012) Kinetic studies of the folding of heterodimeric monellin: evidence for switching between alternative parallel pathways Journal of Molecular Biology, 420 (3). pp. 235-250. ISSN 0022-2836

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jmb.2012.04.019

Abstract

Determining whether or not a protein uses multiple pathways to fold is an important goal in protein folding studies. When multiple pathways are present, defined by transition states that differ in their compactness and structure but not significantly in energy, they may manifest themselves by causing the dependence on denaturant concentration of the logarithm of the observed rate constant of folding to have an upward curvature. In this study, the folding mechanism of heterodimeric monellin [double-chain monellin (dcMN)] has been studied over a range of protein and guanidine hydrochloride (GdnHCl) concentrations, using the intrinsic tryptophan fluorescence of the protein as the probe for the folding reaction. Refolding is shown to occur in multiple kinetic phases. In the first stage of refolding, which is silent to any change in intrinsic fluorescence, the two chains of monellin bind to one another to form an encounter complex. Interrupted folding experiments show that the initial encounter complex folds to native dcMN via two folding routes. A productive folding intermediate population is identified on one route but not on both of these routes. Two intermediate subpopulations appear to form in a fast kinetic phase, and native dcMN forms in a slow kinetic phase. The chevron arms for both the fast and slow phases of refolding are shown to have upward curvatures, suggesting that at least two pathways each defined by a different intermediate are operational during these kinetic phases of structure formation. Refolding switches from one pathway to the other as the GdnHCl concentration is increased.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Monellin; Heterodimeric Protein; Folding Kinetics; Parallel Pathways
ID Code:96224
Deposited On:07 Dec 2012 10:22
Last Modified:07 Dec 2012 10:22

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