Screening of clones containing altered DNA conformations by plasmid topoprofile shift

Ramesh, N. ; Mishra, Rakesh K. (1991) Screening of clones containing altered DNA conformations by plasmid topoprofile shift Journal of Biochemical and Biophysical Methods, 22 (2). pp. 171-175. ISSN 0165-022X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0165-022X(91)90030-Z

Abstract

DNA topoprofile of supercoiled plasmid is influenced by the presence of altered structures in some stretches. We describe a non-radioactive method for screening the clones containing such altered structures.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Supercoiling; Topoprofile; Clone Seletion; DNA Structure, Altered
ID Code:96192
Deposited On:07 Dec 2012 11:45
Last Modified:16 Jan 2013 09:45

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