Swamy, Musti J. ; Marsh, D. ; Anbazhagan, V. ; Ramakrishnan, M. (2002) Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes FEBS Letters, 528 (1-3). pp. 230-234. ISSN 0014-5793
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0014-5793(02)03316-1
Abstract
Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215–2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | BSP-A1; Lipid–Protein Interaction; Lipid Bilayer; Cholesterol Efflux; Phosphatidylcholine; Sphingomyelin |
| ID Code: | 95868 |
| Deposited On: | 07 Dec 2012 05:28 |
| Last Modified: | 07 Dec 2012 05:28 |
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