Inhibition of platelet aggregation by rat globin

Singh, Mahendra P. ; Sinha, Sudhir ; Raghavan, Santhanam A. V. ; Dikshit, Madhu (2002) Inhibition of platelet aggregation by rat globin Thrombosis Research: Vascular Obstruction, Hemorrhage and Hemostasis, 107 (5). pp. 201-207. ISSN 0049-3848

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This study was undertaken to identify and characterize the anti-aggregatory protein factor present in rat polymorphonuclear leukocytes (PMNs) supernatant. Since the purified protein exhibited sequence homology to beta globin, globin was also isolated from rat blood by acid-acetone precipitation and was purified on Superdex-75 column in FPLC. Elution of rat globin on the gel filtration column yielded two peaks of approximately 60 and 30 kDa as observed in the PMNs supernatant. Purity of globin and eluted fractions was further evaluated by SDS-PAGE. Platelet aggregation induced by agonists viz. adenosine-5'-diphosphate (ADP; 2-5 μM), arachidonic acid (AA; 10 μM), A23187 (2.50 μg/ml) was inhibited by globin and the purified fractions. ADP-induced rise in intracellular calcium levels and expression of CD62 on the platelets were reduced by both globin and active fraction of PMNs supernatant. Results obtained suggest that globin or globin-related protein present in the PMNs supernatant inhibits platelet aggregation response.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Platelet Aggregation; Protein Factor Of Neutrophil Supernatant; Rat Globin; CD62; Intracellular Calcium
ID Code:9583
Deposited On:02 Nov 2010 11:51
Last Modified:09 Feb 2011 05:48

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