Dabadghao, S. ; Misra, R. ; Naveed, M. ; Aggarwal, A. (1996) IgM rheumatoid factor estimation by ELISA in seronegative rheumatoid arthritis before and after IgM fractionation: does seronegative RA exist? Rheumatology International, 15 (5). pp. 189-193. ISSN 0172-8172
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Official URL: http://link.springer.com/article/10.1007%2FBF00290...
Related URL: http://dx.doi.org/10.1007/BF00290520
Abstract
We looked for the presence of IgM rheumatoid factor (RF) using an enzyme-linked immunosorbent assay (ELISA) in 25 patients with active seronegative rheumatoid arthritis. In unfractionated sera, 12 patients (48%) were positive for IgM RF (classical), but after IgM fractionation of 23 samples (2 samples were not available) using high performance liquid chromatography for fractionating IgM, 12 patients were positive for RF by ELISA indicatingt the presence of hidden RF. Finally, three patients were labelled as truly seronegative for IgM RF. Classical IgM RF as deteced by ELISA correlated significantly with erosive disease. Hidden RF did not correlate with disease activity or severity in this cross-sectional study, and though its presence was associated with shorter disease duration. this did not reach statistical significance.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Hidden RF; High Performance Liquid Chromatography |
| ID Code: | 95175 |
| Deposited On: | 27 Sep 2013 10:21 |
| Last Modified: | 27 Sep 2013 10:21 |
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