Studies on active site mutants of P-falciparum adenylosuccinate synthetase: Insights into enzyme catalysis and activation

Mehrotra, Sonali ; Mylarappa, B. N. ; Iyengar, Prathima ; Balaram, Hemalatha (2010) Studies on active site mutants of P-falciparum adenylosuccinate synthetase: Insights into enzyme catalysis and activation Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 1804 (10). pp. 1996-2002. ISSN 1570-9639

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.bbapap.2010.07.015

Abstract

Adenylosuccinate synthetase catalyzes a reversible reaction utilizing IMP, GTP and aspartate in the presence of Mg2+ to form adenylosuccinate, GDP and inorganic phosphate. Comparison of similarly liganded complexes of Plasmodium falciparum, mouse and Escherichia coli AdSS reveals H-bonding interactions involving nonconserved catalytic loop residues (Asn429, Lys62 and Thr307) that are unique to the parasite enzyme. Site-directed mutagenesis has been used to examine the role of these interactions in catalysis and structural organization of P. falciparum adenylosuccinate synthetase (PfAdSS). Mutation of Asn429 to Val, Lys62 to Leu and Thr307 to Val resulted in an increase in Km values for IMP, GTP and aspartate, respectively along with a 5 fold drop in the kcat value for N429V mutant suggesting the role of these residues in ligand binding and/or catalysis. We have earlier shown that the glycolytic intermediate, fructose 1,6 bisphosphate, which is an inhibitor of mammalian AdSS is an activator of the parasite enzyme. Enzyme kinetics along with molecular docking suggests a mechanism for activation wherein F16BP seems to be binding to the Asp loop and inducing a conformation that facilitates aspartate binding to the enzyme active site. Like in other AdSS, a conserved arginine residue (Arg155) is involved in dimer crosstalk and interacts with IMP in the active site of the symmetry related subunit of PfAdSS. We also report on the biochemical characterization of the arginine mutants (R155L, R155K and R155A) which suggests that unlike in E. coli AdSS, Arg155 in PfAdSS influences both ligand binding and catalysis.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:P. Falciparum; Adenylosuccinate Synthetase; Site-Directed Mutagenesis; Fructose 1,6 Bisphosphate;
ID Code:94672
Deposited On:18 Oct 2012 09:00
Last Modified:18 Oct 2012 09:00

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