Antileishmanial activity of nano-amphotericin B deoxycholate

Manandhar, Krishna Das ; Yadav, Thakur Prasad ; Prajapati, Vijay Kumar ; Kumar, Subodh ; Rai, Madhukar ; Dube, Anuradha ; Srivastava, Onkar Nath ; Sundar, Shyam (2008) Antileishmanial activity of nano-amphotericin B deoxycholate Journal of Antimicrobial Chemotherapy, 62 (2). pp. 376-380. ISSN 0305-7453

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Objectives The aim of the present study was to compare the efficacy of a nano form of amphotericin B deoxycholate with that of conventional amphotericin B deoxycholate for the treatment of visceral leishmaniasis. Methods We have formulated nanoparticles (10–20 nM) from amphotericin B deoxycholate (1–2 µM) by applying high-pressure (150 argon) milling homogenization and have tested their efficacy in a J774A cell line and in hamsters. Parasite survival and tissue burden in spleen were evaluated for nano-amphotericin B and conventional amphotericin B. Both nano-amphotericin B and conventional amphotericin B were injected intraperitoneally at 5 mg/kg per day for 5 days. Results The inhibition of amastigotes in the splenic tissue with nano-amphotericin B was significantly more than with conventional amphotericin B (92.18% versus 74.57%, P = 0.005). Similarly, the suppression of parasite replication in the spleen was also found to be significant (99.18% versus 97.17%, P = 0.05). In a cytotoxicity test, nano-amphotericin B against the J774A cell line had a CC50 of 12.67 mg/L in comparison with 10.61 mg/L for amphotericin B, far higher than the doses used for ED50. Conclusions Nanoparticles of amphotericin B had significantly greater efficacy than conventional amphotericin B. This formulation may have a favourable safety profile, and if production costs are low, it may prove to be a feasible alternative to conventional amphotericin B.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Visceral Leishmaniasis; Leishmania Donovani; Nanoparticles; Nanomedicine; in Vitro; in Vivo
ID Code:94533
Deposited On:16 Nov 2012 12:38
Last Modified:27 Oct 2015 11:40

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