Lectin-specific targeting of β-glucocerebrosidase to different liver cells via glycosylated liposomes

Das, Pijush K. ; Murray, Gary J. ; Zirzow, Gregory C. ; Brady, Roscoe O. ; Barranger, John A. (1985) Lectin-specific targeting of β-glucocerebrosidase to different liver cells via glycosylated liposomes Biochemical Medicine, 33 (1). pp. 124-131. ISSN 0006-2944

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0006-2944(85)90135-8

Abstract

Galactosylated and mannosylated liposomes were more efficient in transporting liposome-entrapped β-glucocerebrosidase to liver compared to nonglycosylated liposomes. The enzyme entrapped to glycoside-bearing liposomes was found to be cleared at a much faster rate than that entrapped in liposomes having no sugar on their surface. Asialoorosomucoid and hydrolyzed mannan were found to inhibit both the clearance and the uptake of galactosylated and mannosylated liposomes, respectively, supporting involvement of lectin-sugar interaction. Further studies on the uptake of glucocerebrosidase by isolated liver cells revealed that the enzyme entrapped in mannosylated liposomes has much higher affinity for nonparenchymal cells whereas the assimilation of the entrapped enzyme into hepatocytes is clearly favored for liposomes having galactose on their surface.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:93792
Deposited On:27 Jun 2012 11:04
Last Modified:27 Jun 2012 11:04

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