Superoxide release by Zymosan-stimulated Rat Kupffer cells in vitro

Bhatnagar, Rakesh ; Schirmer, Rüdiger ; Ernst, Martin ; Decker, Karl (1981) Superoxide release by Zymosan-stimulated Rat Kupffer cells in vitro European Journal of Biochemistry, 119 (1). pp. 171-175. ISSN 0014-2956

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Kupffer cells were isolated from pronase-perfused rat livers and were maintained as a monolayer culture in a state of high purity and viability. Immediately after contact with zymosan particles, O2 uptake of the Kupffer cells increased fivefold; about 50% of the net oxygen consumed was accounted for as superoxide released into the medium. Concomitantly, a transient burst of luminol-dependent chemiluminescence, an increased activity of NAD(P)H oxidase and a stimulation of the flow of glucose through the hexose monophophate shunt were observed. Chemiluminescence and O2 production were almost completely inhibited by superoxide dismutase and iodoacetate. Zymosan-induced chmiluminesceance was not inhibited in the presence of the non-penetrating thiol reagents, 5,5′-dithio-bis-2-nitrobenzoate and iodoacetyl-sepharose. Iodoacetate acted on the cytosolic glucose-6-phosphate dehydrogenase rather than on NAD(P)H oxidase of the cell membrane.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
ID Code:93507
Deposited On:21 Jun 2012 05:46
Last Modified:21 Jun 2012 05:46

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