A novel MAP-kinase kinase from Candida albicans

Singh, Praveen ; Ghosh, Sharmistha ; Datta, Asis (1997) A novel MAP-kinase kinase from Candida albicans Gene, 190 (1). pp. 99-104. ISSN 0378-1119

Full text not available from this repository.

Official URL: http://linkinghub.elsevier.com/retrieve/pii/S03781...

Related URL: http://dx.doi.org/10.1016/S0378-1119(96)00758-5


A putative MAP-kinase kinase-encoding gene, CaSTE7, was isolated from Candida albicans by complementation of ste7 and stell mutants of the pheromone signal-transduction pathway of Saccharomyces cerevisiae. The nucleotide (nt) sequence revealed an ORF of 1767 nt encoding a putative protein of 589 amino acids (aa). CaSTE7 has a strong homology with MAP-kinase kinase STE7 of S. cerevisiae, the kinase domain having 45% homology with that of STE7. The deduced aa sequence contained all eleven consensus kinase subdomains found in MAP-kinase kinases. It can suppress the mating defect of ste5, stell, ste7, and fus3 kssl double mutants, but it cannot bypass the ste12 mutation. CaSTE7 behaves as a hyperactive allele of STE7, suppressing the mating defects of the pheromone signal-transduction pathway by constitutively stimulating STE12, and hence STE12-dependent processes.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Saccharomyces Cerevisiae; Pheromone; Signal Transduction
ID Code:9336
Deposited On:02 Nov 2010 12:27
Last Modified:07 Feb 2011 08:46

Repository Staff Only: item control page