The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins

Pandey, Aarti ; Choudhary, Mani Kant ; Bhushan, Deepti ; Chattopadhyay, Arnab ; Chakraborty, Subhra ; Datta, Asis ; Chakraborty, Niranjan (2006) The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins Journal of Proteome Research, 5 (12). pp. 3301-3311. ISSN 1535-3893

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Nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. The yeast nuclear proteome corresponds to about one-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number of diverse functions. In an attempt to understand the complexity of plant nuclear proteins, we have developed a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis (2-DE). Approximately, 600 protein spots were detected, and LC-ESI-MS/MS analyses led to the identification of 150 proteins that have been implicated in a variety of cellular functions. The largest percentage of the identified proteins was involved in signaling and gene regulation (36%), while 17% were involved in DNA replication and transcription. The chickpea nuclear proteome indicates the presence of few new nuclear proteins of unknown functions vis-a-vis many known resident proteins. To the best of our knowledge, this is the first report of a nuclear proteome of an unsequenced genome.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
Keywords:Chickpea; Nuclear Proteins; Proteome; 2-DE; Mass Spectrometry
ID Code:9312
Deposited On:02 Nov 2010 12:31
Last Modified:07 Feb 2011 06:34

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