Lipozyme deactivation by butanol and temperature

Gandhi, Neena N. ; Sawant, Sudhirprakash B. ; Joshi, Jyeshtharaj B. ; Mukesh, D. (1995) Lipozyme deactivation by butanol and temperature Enzyme and Microbial Technology, 17 (4). pp. 373-380. ISSN 0141-0229

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The Mucor miehei lipase, Lipozyme, has been found to be vulnerable to temperature-induced deactivation. This thermodeactivation is further aggravated by exposure to butanol. The deleterious effect of the latter on the lipase is a function of its concentration in the aqueous phase containing the enzyme. At 50°C, it was found that a crucial butanol concentration (0.81 m) exists, up to which there is only a slight decrease (25%) in lipase activity. Beyond this, the fall in activity is rapid, with total deactivation at 0.99 m concentration. The deactivation kinetics of the lipase are modeled using a series-type mechanism.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Lipozyme; Deactivation; Butanol; Temperature; Series-type Mechanism
ID Code:93003
Deposited On:08 Jun 2012 08:36
Last Modified:08 Jun 2012 08:36

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