Sawant, S. B. ; Sikdar, S. K. ; Joshi, J. B. (1990) Hydrodynamics and mass transfer in two-phase aqueous extraction using spray columns Biotechnology and Bioengineering, 36 (2). pp. 109-115. ISSN 0006-3592
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/bit.260...
Related URL: http://dx.doi.org/10.1002/bit.260360202
Abstract
Spray columns can be used to isolate and purify proteins using the two-phase aqueous extraction technique based on polyethylene glycol (PEG) and dextran. The fractional dispersed phase (PEG) holdup and overall mass transfer coefficients were measured in a 9.7 mm i.d. spray column. We found that the dispersed phase holdup increased with increasing PEG phase velocity. The overall mass transfer coefficients for bovine serum albumin, normalized for the PEG holdup, were found to be independent of the PEG phase velocity. This result was expected, since true mass transfer coefficients do not vary with phase velocity.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 92989 |
| Deposited On: | 08 Jun 2012 08:32 |
| Last Modified: | 08 Jun 2012 08:32 |
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