Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p

Subba Rao, G. ; Bachhawat, Anand K. ; Gupta, C. (2002) Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p Functional and Integrative Genomics, 1 (6). pp. 357-366. ISSN 1438-793X

[img]
Preview
PDF - Publisher Version
5MB

Official URL: http://www.springerlink.com/index/G115TNLH0VKCL2W3...

Related URL: http://dx.doi.org/10.1007/s10142-001-0040-4

Abstract

The ATP-binding cassette (ABC) transporters are a large family of proteins responsible for the translocation of a variety of compounds across the membranes of both prokaryotes and eukaryotes. The inter-protein and intra-protein interactions in these traffic ATPases are still only poorly understood. In the present study we describe, for the first time, an extensive yeast two-hybrid (Y2H)-based analysis of the interactions of the cytoplasmic loops of the yeast pleiotropic drug resistance (Pdr) protein, Pdr5p, an ABC transporter of Saccharomyces cerevisiae. Four of the major cytosolic loops that have been predicted for this protein [including the two nucleotide-binding domain (NBD)-containing loops and the cytosolic C-terminal region] were subjected to an extensive inter-domain interaction study in addition to being used as baits to identify potential interacting proteins within the cell using the Y2H system. Results of these studies have revealed that the first cytosolic loop (CL1) - containing the first NBD domain - and also the C-terminal region of Pdr5p interact with several candidate proteins. The possibility of an interaction between the CL1 loops of two neighboring Pdr5p molecules was also indicated, which could possibly have implications for dimerization of this protein.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Yeast Two-hybrid System; ABC Transporter; Pdr5p; NBDI
ID Code:924
Deposited On:25 Sep 2010 04:28
Last Modified:16 May 2016 12:06

Repository Staff Only: item control page