Gupta, Naveen ; Reddy, Vanga Shiva ; Maiti, Sankar ; Ghosh, Amit (2000) Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic bacillus sp. Strain NG-27 Applied and Environmental Microbiology, 66 (6). pp. 2631-2635. ISSN 0099-2240
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Official URL: http://aem.asm.org/content/66/6/2631.abstract
Related URL: http://dx.doi.org/10.1128/?AEM.66.6.2631-2635.2000
Abstract
Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70°C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 β -glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Society for Microbiology. |
| ID Code: | 91875 |
| Deposited On: | 25 May 2012 05:15 |
| Last Modified: | 25 May 2012 05:15 |
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