Purification and properties of DNA gyrase from V. cholerae

Mukhopadhyay, G. ; Ghosh, A. (1991) Purification and properties of DNA gyrase from V. cholerae Biochemical Journal, 280 (3). pp. 797-800. ISSN 0264-6021

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Official URL: http://www.biochemj.org/bj/280/bj2800797.htm

Abstract

The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.

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