Mathur, R. ; Balasubramanian, A. S. (1984) Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase Biochemical Journal, 222 (1). pp. 261-264. ISSN 0264-6021
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Official URL: http://www.biochemj.org/bj/222/0261/bj2220261_brow...
Abstract
A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Portland Press. |
| ID Code: | 91617 |
| Deposited On: | 22 May 2012 12:16 |
| Last Modified: | 22 May 2012 12:16 |
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