Replication of Epstein-Barr virus DNA in lymphoblastoid cells treated for extended periods with acyclovir

Colby, Brenda M. ; Shaw, James E. ; Datta, Alok K. ; Pagano, Joseph S. (1982) Replication of Epstein-Barr virus DNA in lymphoblastoid cells treated for extended periods with acyclovir American Journal of Medicine, 73 (1). pp. 77-81. ISSN 0002-9343

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000293...

Related URL: http://dx.doi.org/10.1016/0002-9343(82)90068-7

Abstract

An Epstein-Barr virus (EBV)-producing human lymphoblastoid cell line (P3HR-1) was characterized after prolonged exposure to 9-(2-hydroxyethoxymethyl)guanine (acyclovir). P3HR-1 cells grown for 11 months in the presence of acyclovir had the same number of EBV genomes (approximately 15/cell) as did short-term acyclovir-treated cells. After removal of the drug, the number of EBV genome equivalents of long-term acyclovir-treated cultures (15/cell) returned to P3HR-1 control levels (340/cell). Indirect immunofluorescence studies demonstrated reduced percentages of long-term (6 to 12 months) acyclovir-treated P3HR-1 cells expressing both EBV early antigens (EA) and viral capsid antigens (VCA). This contrasts with short-term acyclovir-treated cultures where EA is expressed at normal levels even though VCA expression is reduced. Restriction fragment patterns of the total EBV DNA from both short and long-term acyclovir-treated cultures were identical with the exception of two fragments present in short- but not long-term acyclovir-treated cultures. EBV-associated DNA polymerase was present in long-term acyclovir-treated cultures with the level of activity, as determined by assay in vitro, comparable to that in untreated P3HR-1 cells. The polymerase activity in the treated cells was sensitive in vitro to acyclovir triphosphate, N-ethyimaleimide (NEM), and phosphonoacetic acid (PAA). These studies demonstrate that acyclovir inhibits the EBV-productlve cycle in P3HR-1 cells continuously during extended periods of treatment.

Item Type:Article
Source:Copyright of this article belongs to Association of Professors of Medicine.
ID Code:9069
Deposited On:29 Oct 2010 11:45
Last Modified:30 May 2011 06:53

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