Characterization of the dengue virus-induced helper cytokine

Chaturvedi, Pratibha ; Mukherjee, Rinee ; Chaturvedi, U. C. ; Mathur, Asha (1992) Characterization of the dengue virus-induced helper cytokine International Journal of Experimental Pathology, 73 . pp. 263-272. ISSN 0959-9673

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Dengue type 2 virus (DV) induces a subpopulation of T lymphocytes of mouse spleen to secrete a soluble helper cytokine (HF) which enhances the DV-specific IgM antibody plaque forming cells (PFC). The present study undertaken to purify and characterize HF shows that it can be purified by low pressure liquid chromatography (LPLC) using Sephacryl S-200 column. HF consisted of two subunits, having a Mr of 6 5-68 kDa on SDS-PAGE, and both had similar activity. The isoelectric point of HF was 6.5. HF-specific antisera (HFAS) raised in mice neutralized the activity ofHF in mice, reacted with it in a Western blot assay, and bound HF in an immunosorbent column. HF bound to DV-antigen in an immunosorbent column and enhanced only the DV-specific PFC. HF had no effect on PFC against heterologous antigens such as Japanese encephalitis virus, Coxsackie B4 virus or sheep red blood cells. HF generated in mice of H-2k haplotype, enhanced DV-specific PFC in the same strain of mice but had no effect on that in the H-2d or H-2q haplotype strains of mice. Thus, DV-induced HF with a Mr of 6 5-6 8 kDa, antigen-specificity and genetic-restriction differs from most of the similarly acting cytokines but appears similar to the cell-free form of T cell receptor α, β dimer.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Helper Cytokine; Dengue Virus; Antigen-specific; Genetic Restriction
ID Code:90539
Deposited On:10 May 2012 10:33
Last Modified:25 Jun 2012 10:41

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