Binding of macrophages and B lymphocytes mediated by dengue virus antigen and the virus-induced helper cytokine

Rizvi, N. ; Chaturvedi, P. ; Chaturvedi, U. C. (1993) Binding of macrophages and B lymphocytes mediated by dengue virus antigen and the virus-induced helper cytokine International Journal of Experimental Pathology, 74 . pp. 187-194. ISSN 0959-9673

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Official URL: http://onlinelibrary.wiley.com/journal/10.1111/%28...

Abstract

Macrophage (Mφ) process dengue type 2 virus (DV) antigen and present it to B cells leading to their clonal expansion and transmit DV-specific helper cytokine (HF) signal to B cells. The present study was, therefore, undertaken to investigate the binding of DV or HF-pulsed Mφ with 51Crlabelled B lymphocytes. The binding was dependent on the number of DV antigen positive Mφ or dose of HF, period (peak with DV at 24 h and with HF at 4 h) and temperature of incubation. The binding was significantly increased by treating DV-pulsed Mφ with DV-specific helper T cells or HF. Glutaraldehyde fixed Mφ (which do not process DV antigen (Rizvi et a/. 1989)) had poor binding capacity. The binding was DV- or HF-specific as it was inhibited by treatment of Mφ with specific antisera. The findings thus show that Mφ and B lymphocytes are actively bridged via DV antigen and HF to effectively transmit the antigen-specific signal.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Dengue Virus; Helper Cytokine; Macrophage-lymphocyte Binding
ID Code:90536
Deposited On:10 May 2012 10:33
Last Modified:10 May 2012 10:33

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