A study of dengue imported to Kuwait during 1997-1999

Mustafa, A. S. ; Elbishbishi, E. A. ; Grover, S. ; Pacsa, A. S. ; Al-Enezi, A. A. ; Chaturvedi, U. C. (2001) A study of dengue imported to Kuwait during 1997-1999 Acta Virologica, 45 (2). pp. 125-128. ISSN 0001-723X

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This study was carried out on sera from 210 patients in Kuwait in 1997-99. All of the patients were suffering from febrile illness and had recently visited dengue- (DEN) endemic areas. The sera were screened for DEN virus by inoculation into cultures of the Aedes albopictus cell clone C6/36 (virus isolation) and by IgM capture ELISA (detection of DEN virus-specific IgM antibodies). In the cell cultures, DEN virus could not be isolated from any of the patients' sera. However, sera from 19 patients were positive for DEN virus-specific IgM antibodies. All these 19 sera were tested for the presence of DEN virus-specific RNA by reverse transcription-PCR (RT-PCR) using DEN virus types-common (consensus) primers. In addition, the type of DEN virus was identified by using type-specific primers in a semi-nested PCR. The results showed that 2 of the 19 patients were infected with DEN virus type 2. This report of 19 patients with serological evidence of DEN infection indicates that imported DEN is a real threat to Kuwait, a country non-endemic for DEN but with a large portion of the population vacationing in DEN-hyperendemic areas during the peak DEN season and then returning to Kuwait.

Item Type:Article
Source:Copyright of this article belongs to Academic Electronic Press.
ID Code:90518
Deposited On:10 May 2012 10:38
Last Modified:10 May 2012 10:38

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