Proteome maps of flood-tolerant FR 13A and flood-sensitive IR 54 rice types depicting proteins associated with O₂-deprivation stress and recovery regimes

Abstract

Rice crop, particularly in lowland ecosystem, is severely affected by flooding stress. To genetically engineer rice with improved flooding tolerance, it is important to gain an understanding of the proteins that constitute its flooding-stress response. A major component of the flooding stress is a decline in availability of O₂. Proteomics approach is a powerful tool for analysing global changes in protein profiles. We have constructed proteome maps of flood-sensitive IR 54 and floodtolerant FR 13A rice types corresponding to several time points during their response to O₂ deprivation stress. Several up- and down-regulated proteins have been identified by 1D and 2D protein gel electrophoresis followed by silver staining. Based on peptide sequence analysis, we indicate that sucrose synthase, glyceraldehyde 3-phosphate dehydrogenase, UDP-glucose- 6-dehydrogenase and asparagine synthetase are implicated in rice flooding-stress response.