Chandrashekaran, Siddamadappa ; Babu, Padmanabhan ; Nagaraja, Valakunja (1999) Purification and characterization of restriction endonuclease BasI from Bacillus species The Journal of Biochemistry, Molecular Biology and Biophysics, 3 . pp. 225-229. ISSN 1025-8140
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Abstract
After screening a variety of bacteria from soil for the DNA cleavage activity, a type II restriction endonuclease, BasI was isolated and characterized from a Bacillus species. Using the purified enzyme the recognition sequence was determined. The enzym recognizes 5'-CCANNNN↓NTGG-3'sequence and cleaves as indicated. The enzyme functions efficiently at low lonic strength, pH rane and at the optimum temperature of 30° C.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Group. |
| Keywords: | Testriction Endonuclease; Bacillus Species; Lsoschizomer |
| ID Code: | 89565 |
| Deposited On: | 28 Apr 2012 14:28 |
| Last Modified: | 03 Jul 2012 09:42 |
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