Chandrashekaran, Siddamadappa ; Shankar, Aparna Bhavani ; Babu, Padmanabhan ; Paul, Bindu Diana ; Nagaraja, Valakunja (1999) Identification and characterization of a type II restriction endonuclease, Str1 from Streptomyces thermodiastaticus Current Science, 77 (2). pp. 273-276. ISSN 0011-3891
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Abstract
A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5' -C↓TCGAG-3' as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.
| Item Type: | Article |
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| Source: | Copyright of this article belongs to Current Science Association. |
| ID Code: | 89555 |
| Deposited On: | 28 Apr 2012 14:30 |
| Last Modified: | 19 May 2016 04:05 |
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