An ESR study of metal ion-guanosine monophosphate interaction

Podder, S. K. ; Kasthuri Rengan, S. ; Navalgund, Ranganath (1974) An ESR study of metal ion-guanosine monophosphate interaction Journal of Magnetic Resonance(1969), 15 (2). pp. 254-261. ISSN 1090-7807

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The addition of guanosine 5-monophosphate (5'-GMP) to an aqueous solution of Mn2+ ions results in a decrease in ESR signal intensity and an increase in line-width of Mn2+ ions. This can be interpreted in terms of stepwise formation of outersphere and inner-sphere complexes as Mn2+(H2O)6 +5'-GMP ⇆ K1[ Mn2+(H2O)6, 5'-GMP] ⇆K1[Mn2+(H2O)5, 5'-GMP] +H2O. From the measurement of ESR signal intensity and linewidth as a function of concentration of 5'-GMP at room temperature (23°C ), the stability constants K1 and K2 are calculated as 180 M-1 and 1.14, respectively. When Mg2+ is added to a mixture of Mn2+ and 5'-GMP, ESR signal intensity increases, presumably due to the replacement of Mn2+ by Mg2+ in the complex. From the variation of ESR signal intensity as a function of concentration of Mg2+, the product K1K2 for the magnesium complex is calculated as 125 M-1. This difference in stability constants may indicate that both phosphate group and guanine base are involved in the formation of Mn2+-5'-GMP complex.

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Source:Copyright of this article belongs to Elsevier Science.
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Deposited On:26 Apr 2012 13:02
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