Neutrophil extracellular traps contain mitochondrial as well as nuclear DNA and exhibit inflammatory potential

Keshari, Ravi S. ; Jyoti, Anupam ; Kumar, Sachin ; Dubey, Megha ; Verma, Anupam ; Srinag, Bangalore S. ; Krishnamurthy, Hanumanthappa ; Barthwal, Manoj K. ; Dikshit, Madhu (2012) Neutrophil extracellular traps contain mitochondrial as well as nuclear DNA and exhibit inflammatory potential Cytometry, Part A, 81A (3). pp. 238-247. ISSN 1552-4922

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/cyto.a....

Related URL: http://dx.doi.org/10.1002/cyto.a.21178

Abstract

Neutrophils expel extracellular traps (NETs) to entrap and exterminate the invaded micro-organisms. Acute/chronic inflammatory disorders are often observed with aberrantly enhanced NETs formation and high nitric oxide (NO) availability. Recent study from this laboratory demonstrated release of NETs from human neutrophils following treatment with SNP or SNAP. This study is an extension of our previous finding to explore the extracellular bacterial killing, source of DNA in the expelled NETs, their ability to induce proinflammatory cytokines release from platelets/THP-1 cells, and assessment of NO-mediated free radical formation by using a consistent NO donor, DETA-NONOate. NO-mediated NETs exhibited extracellular bacterial killing as determined by colony forming units. NO-mediated NETs formation was due to the activation of NADPH oxidase and myeloperoxidase. NO- or PMA-mediated NETs were positive for both nuclear and mitochondrial DNA as well as proteolytic enzymes. Incubation of NETs with human platelets enhanced the release of IL-1β and IL-8, while with THP-1 cells, release of IL-1β, IL-8, and TNFα was observed. This study demonstrates that NO by augmenting enzymatic free radical generation release NETs to promote extracellular bacterial killing. These NETs were made up of mitochondrial and nuclear DNA and potentiated release of proinflammatory cytokines.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Nitric Oxide; Neutrophil Extracellular Traps; NADPH-oxidase; Myeloperoxidase; Inflammatory Cytokine; Mitochondrial and Nuclear DNA
ID Code:89056
Deposited On:30 Mar 2012 16:15
Last Modified:30 Mar 2012 16:15

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