Nithianandam, V. S. ; Srinivasan, K. S. V. ; Joseph, K. T. ; Santappa, M. (1981) Immobilisation of trypsin on poly (menthl methacrylate-Co-acryloylamino-2-methylpropanesulfonic acid) Die Angewandte Makromolekulare Chemie, 182 (8). pp. 2193-2200. ISSN 0003-3146
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/macp.19...
Related URL: http://dx.doi.org/10.1002/macp.1981.021820808
Abstract
Trypsin was immobilized on a copolymer of methyl methacrylate and 2-acryloylamino-2-methylpropanesulfinic acid. The resulting immobilized trypsin shows 25% of the soluble enzyme specific activity. The pH optimum of the immobilized trypsin is shifted to a higher value by 1,1 units. The pH stability of the immobilized trypsin is better than that of the soluble enzyme, whereas the temperature stability of the soluble and the immobilized enzyme is almost identical.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 88866 |
| Deposited On: | 29 Mar 2012 14:27 |
| Last Modified: | 29 Mar 2012 14:27 |
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