Factors influencing the production cellular acid proteinase by Aspergillius fumigatus FRESIMI No. 215396

Abstract

Extracellular acid proteinases of many species of Aspergillus and Penicillium were comparatively studied. Aspergillus fumigatus was found to yield the highest enzyme activity. The influence of several cultural conditions viz., incubation period and temperature, pH of the medium, aeration and depth of the medium, on the production of extracellular acid proteinase by A. fumigatus was systematically investigated. The maximum concentration of the enzyme was obtained when the culture medium of pH 9.5 was incubated at 31°C for a period of 3 days. The important nutritional factors which affect the production of acid proteinase viz., the effects of different carbohydrates, different forms of organic and inorganic nitrogen, different metallic salts in the culture medium were critically investigated. Based on the results obtained, the composition of the medium for the maximum production of extracellular acid proteinase by A. fumigatus has been suggested.