Factors affecting the extraction of from Jawasee

Abstract

Various experimental procedures for the extraction of protease from jawasee powder were adopted to ascertain optimum conditions for leaching- deionized distilled water at pH 7.0 and 37° C was found to extract maximum enzyme in 24 hours. The jawasee powder is optimally active at pH 6.0 with 2.5% egg albumin as a substrate. Sodium chloride at higher concentration inhibits the enzyme considerably. Strong inhibitory action was observed in case of 4 preservatives, namely, para-chloro-meta-cresol, sodium pentachlorophenate, phenyl mercuric nitrate and sodium tri-chlorophenate.