Ehtesham, N. Z. ; Talwar, G. P. ; Ali, A. ; Hasnain, S. E. (1990) Molecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism Indian Journal of Biochemistry & Biophysics, 27 (5). pp. 275-279. ISSN 0301-1208
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Abstract
A approximately 400 bp HaeIII human genomic satellite DNA band was cloned into pUC18 to construct a partial library. A fragment of bacteriophage M13 containing a sequence homologous to the human minisatellite core was cloned in pUC18 and was used as a probe to isolate a approximately 350 bp human satellite clone (pTRF5.6) from the partial library. Other clones from this library showed a wide variation in terms of size and hybridization to the pTRF5.6 clone. Human DNA from different individuals was digested with restriction enzymes, Southern transferred and probed with TRF5.6. Individual-specific complex pattern of DNA bands was produced. TRF5.6, therefore, could be useful as a probe for detecting genetic polymorphism.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 88627 |
| Deposited On: | 29 Mar 2012 09:24 |
| Last Modified: | 29 Mar 2012 09:24 |
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