Simultaneous synthesis of enzymatically active luciferase and biologically active β subunit of human chorionic gonadotropin in caterpillars infected with a recombinant baculovirus

Jha, Prakash K. ; Pal, Rahul ; Nakhai, Bita ; Sridhar, Padma ; Hasnain, Seyed E. (1992) Simultaneous synthesis of enzymatically active luciferase and biologically active β subunit of human chorionic gonadotropin in caterpillars infected with a recombinant baculovirus FEBS Letters, 310 (2). pp. 148-152. ISSN 0014-5793

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0014-5793(92)81317-F

Abstract

The β subunit of human chorionic gonadotropin (βhCG), a secretory and extensively glycosylated hormone, and firefly luciferase, a non-secretory enzyme, were simultaneously synthesized in Spodoptera larvae upon infection with a dual expression recombinant baculovirus, vAcβhCG-luc. Luciferase was retained predominantly in the body tissue while βhCG was secreted into the hemolymph of infected larvae. Both the proteins were similar to their authentic counterparts in terms of immunoreactivity and bioactivity. The caterpillar-derived recombinant hCG exhibited reduced electrophoretic mobility on SDS-PAGE and increased biological activity as compared to the hCG expressed in insect cells in culture. The implications of using the larval system for expressing an extensively glycosylated protein are discusced.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:β Subunit of Human Chorionic Gonadotropin; βhCG; Baculovirus System; Dual Expression; Luciferase; Recombinant DNA; Secretion; Glycosylation
ID Code:88620
Deposited On:29 Mar 2012 09:27
Last Modified:29 Mar 2012 09:27

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