Regions of high antigenicity within the hypothetical PPE major polymorphic tandem repeat open-reading frame, Rv2608, show a differential humoral response and a low T cell response in various categories of patients with tuberculosis

Chakhaiyar, Prachee ; Nagalakshmi, Yellajosyula ; Aruna, Bandi ; Murthy, Kolluri J. R. ; Katoch, Vishwa M. ; Hasnain, Seyed E. (2004) Regions of high antigenicity within the hypothetical PPE major polymorphic tandem repeat open-reading frame, Rv2608, show a differential humoral response and a low T cell response in various categories of patients with tuberculosis The Journal of Infectious Diseases, 190 (7). pp. 1237-1244. ISSN 0022-1899

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Official URL: http://jid.oxfordjournals.org/content/190/7/1237.a...

Related URL: http://dx.doi.org/10.1086/423938

Abstract

The function of the PE/PPE families of proteins, which represent ~10% of the coding capacity of the Mycobacterium tuberculosis genome, has remained relatively unknown. We earlier described a PPE family member, Rv2430c, as an immunodominant antigen. We now report another PPE family gene, Rv2608, a member of the major polymorphic tandem repeat subfamily, for its ability to elicit a high humoral and a low T cell response. Rv2608 was also found to be polymorphic in different clinical isolates of M. tuberculosis, as determined by polymerase chain reaction-restriction fragment-length polymorphism analysis. A total of 51 clinically confirmed patients with tuberculosis (TB), belonging to 3 different categories-fresh infection (n = 22), relapsed infection (n = 21), and extrapulmonary infection (n = 8)-and 10 healthy control subjects were included in the study. Recombinant Rv2608 protein showed positive reactivity to patients serum samples. Enzyme-linked immunosorbent assays and T cell-proliferation assays with synthetic peptides corresponding to predicted regions of high antigenicity showed a predominantly humoral response in patients with relapsed TB. We additionally identified the Gly-X-Gly-Asn-X-Gly repeat motifs as being primarily responsible for eliciting a humoral immune response.

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