Ghosh, Sudip ; Rasheedi, Sheeba ; Rahim, Sheikh Showkat ; Banerjee, Sharmistha ; Choudhary, Rakesh Kumar ; Chakhaiyar, Prachee ; Ehtesham, Nasreen Z. ; Mukhopadhyay, Sangita ; Hasnain, Seyed E. (2004) Method for enhancing solubility of the expressed recombinant proteins in Escherichia coli Biotechniques, 37 (3). pp. 418-423. ISSN 0736-6205
Full text not available from this repository.
Official URL: http://www.biotechniques.com/multimedia/archive/00...
Abstract
The production of correctly folded protein in Escherichia coli is often challenging because of aggregation of the overexpressed protein into inclusion bodies. Although a number of general and protein-specific techniques are available, their effectiveness varies widely. We report a novel method for enhancing the solubility of overexpressed proteins. Presence of a dipeptide, glycylglycine, in the range of 100 mM to 1 M in the medium was found to significantly enhance the solubility (up to 170-fold) of the expressed proteins. The method has been validated using mycobacterial proteins, resulting in improved solubilization, which were otherwise difficult to express as soluble proteins in E. coli. This method can also be used to enhance the solubility of other heterologous recombinant proteins expressed in a bacterial system.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Informa plc. |
| ID Code: | 88593 |
| Deposited On: | 29 Mar 2012 09:40 |
| Last Modified: | 29 Mar 2012 09:40 |
Repository Staff Only: item control page
