Association of cytolethal distending toxin locus cdtB with enteropathogenic Escherichia coli isolated from patients with acute diarrhea in Calcutta, India

Pandey, Mritunjay ; Khan, Asis ; Das, Suresh C. ; Sarkar, Bhaswati ; Kahali, Soumen ; Chakraborty, Subhra ; Chattopadhyay, Santanu ; Yamasaki, Shinji ; Takeda, Yoshifumi ; Balakrish Nair, G. ; Ramamurthy , T. (2003) Association of cytolethal distending toxin locus cdtB with enteropathogenic Escherichia coli isolated from patients with acute diarrhea in Calcutta, India Journal of Clinical Microbiology, 41 (11). pp. 5277-5281. ISSN 0095-1137

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Official URL: http://jcm.asm.org/content/41/11/5277.abstract

Related URL: http://dx.doi.org/10.1128/JCM.41.11.5277-5281.2003

Abstract

Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These isolates were identified as being enteropathogenic E. coli (EPEC). In a retrospective study using a probe hybridization assay, 6 of 138 EPEC strains were found to harbor the cdtB locus. cdtB-positive isolates mostly belong to the O86a and O127a serogroups, with the former being associated with higher expression of CDT. Pulsed-field gel electrophoresis profiles showed that the EPEC strains harboring cdtB strains are genetically diverse.

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