Shigella dysenteriae type 1-specific bacteriophage from environmental waters in Bangladesh

Faruque, Shah M. ; Chowdhury, Nityananda ; Khan, Rasel ; Rubayet Hasan, M. ; Nahar, Jebun ; Johirul Islam, M. ; Yamasaki, Shinji ; Ghosh, A. N. ; Balakrish Nair, G. ; Sack, David A. (2003) Shigella dysenteriae type 1-specific bacteriophage from environmental waters in Bangladesh Applied and Environmental Microbiology, 69 (12). pp. 7028-7031. ISSN 0099-2240

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Official URL: http://aem.asm.org/content/69/12/7028.short

Related URL: http://dx.doi.org/10.1128/AEM.69.12.7028-7031.2003

Abstract

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface water samples from Bangladesh that specifically lyses strains of S. dysenteriae type 1. This phage, designated SF-9, belongs to the Podoviridae family and has a 41-kb double-stranded DNA genome. Further screening of water samples for the prevalence of the phage revealed 9 of 71 (12.6%) water samples which were positive for the phage. These water samples were also positive in PCR assays for one or more S. dysenteriae type 1-specific genes, including ipaBCD and stx1, and live S. dysenteriae type 1 was isolated from three phage-positive samples. The results of this study suggest that phage SF-9 may have epidemiological applications in tracing the presence of S. dysenteriae type 1 in environmental waters.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:88282
Deposited On:27 Mar 2012 12:56
Last Modified:27 Mar 2012 12:56

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