Studies on transfer RNA from mycobacteria

Dileep Deobagkar, N. ; Gopinathan, K. P. (1978) Studies on transfer RNA from mycobacteria Canadian Journal of Microbiology, 24 (6). pp. 693-702. ISSN 0008-4166

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Active preparations of tRNA and aminoacyl-tRNA synthetases have been isolated from exponentially growing cells of Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. Though the aminoacyl-tRNA synthetases of older cells retain their activity, the tRNAs seem to undergo modification and show poorer activity. The mycobacterial enzyme preparations catalyse homologous and heterologous aminoacylation between tRNA from the two species (M. smegmatis and M. tuberculosis H37Rv) or from Escherichia coli, with equal efficiency; tRNA samples from eukaryotic cells (yeast and rat liver) do not serve as substrates for the mycobacterial synthetases. The analytical separation of the different amino acid specific tRNAs from M. smegmatis resembles the pattern found in other bacteria. Purification of valine- (three species) and methionine-specific tRNAs (two species) to 70-80% purity has been accomplished by using column-chromatographic techniques. Of the two species of tRNAMet, one can be formylated in the presence of formyl tetrahydrofolate and the transformylase from mycobacteria.

Item Type:Article
Source:Copyright of this article belongs to NRC Research Press.
ID Code:88074
Deposited On:27 Mar 2012 08:04
Last Modified:27 Mar 2012 08:04

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