A leucyl transfer RNA specified by the amber supressor gene Su6+

Gopinathan, K. P. ; Garen, Alan (1970) A leucyl transfer RNA specified by the amber supressor gene Su6+ Journal of Molecular Biology, 47 (3). pp. 393-401. ISSN 0022-2836

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0022-2836(70)90310-4


The species of leucyl-tRNA responsible for the amber suppressor activity of the Su6+ gene was partially purified (60-fold increase in specific suppressor activity) by chromatography on benzoylated DEAE-cellulose. The homologous species of leucyl-tRNA (without suppressor activity) was similarly purified from an Su6- strain. Each of the species of tRNA accounts for about 5% of the total leucine-acceptor activity. The binding to ribosomes of leucyl-suppressor tRNA from the Su6+ strain is stimulated by the amber codon UAG but not by the leucine codon UUG. The homologous leucyl-tRNA from the Su6- strain shows the opposite codon specificity, binding to ribosomes with UUG but not with UAG, These results suggest that in the Su6+ strain, the anticodon CAA of one species of leucyl-tRNA has been changed to CUA as a result of the suppressor mutation in the Su6 gene. There is another species of leucyl-tRNA which also binds to ribosomes with UUG; this species is not affected by the suppressor mutation and therefore remains available in the Su6+ strain for the translation of UUG.

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ID Code:88067
Deposited On:27 Mar 2012 08:02
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