Levuglandin E2 crosslinks proteins

Iyer, R. S. ; Ghosh, S. ; Salomon, R. G. (1989) Levuglandin E2 crosslinks proteins Prostaglandins & Other Lipid Mediators, 37 (4). pp. 471-480. ISSN 0090-6980

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0090-6980(89)90096-8


Levuglandin E2 (LGE2), a γ -ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, causes extensive intermolecular crosslinking of ovalbumin at pH 6 or pH 7 and 37° C. The time dependence of protein oligomerization is monitored by SDS-PAGE. Effects of pH and concentration on the extent of LGE2-induced crosslinking are examined. The efficacy of LGE2 for inducing crosslinking is compared with other oxidative metabolites of arachidonic acid (AA), including the prostaglandins PGE2, PGD2, PGA2, PGB2, and PGF2α , as well as malondialdehyde and E-4-hydroxy-non-2-enal. LGE2 is orders of magnitude more effective in crosslinking protein than any other cyclooxygenase or lipoxygenase metabolite of AA tested.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:88015
Deposited On:26 Mar 2012 13:52
Last Modified:26 Mar 2012 13:52

Repository Staff Only: item control page