Das Guptaa, S. ; Ghosha, D. K. ; Majumdera, H. K. (1991) A cloned kinetoplast DNA mini-circle fragment from a Leishmania spp. specific for post-kala-azar dermal Leishmaniasis strains Parasitology, 102 (2). pp. 187-191. ISSN 0031-1820
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Official URL: http://journals.cambridge.org/action/displayAbstra...
Related URL: http://dx.doi.org/10.1017/S0031182000062478
Abstract
DNA-DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Cambridge University Press. |
| Keywords: | Leishmania spp; ; Mini-circle; Hybridization; DNA Probes |
| ID Code: | 87809 |
| Deposited On: | 22 Mar 2012 07:52 |
| Last Modified: | 22 Mar 2012 07:52 |
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