Detection of molecular variability in rice tungro bacilliform viruses from India using polymerase chain reaction-restriction fragment length polymorphism

Joshi, Rekha ; Kumar, Vishnu ; Dasgupta, Indranil (2003) Detection of molecular variability in rice tungro bacilliform viruses from India using polymerase chain reaction-restriction fragment length polymorphism Journal of Virological Methods, 109 (1). pp. 89-93. ISSN 0166-0934

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0166-0934(02)00290-2

Abstract

Rice tungro bacilliform virus (RTBV) with rice tungro spherical virus (RTSV) causes the destructive tungro disease of rice. In order to ascertain the molecular variability of RTBV in India, primers were designed to amplify a polymorphic DNA fragment of the virus. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis on a number of field isolates indicated mixed infections and molecular heterogeneity in the viral genome.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Rice Tungro Bacilliform Virus; Polymerase Chain Reaction; Strain Variability; Restriction Fragment Length Polymorphism
ID Code:86670
Deposited On:12 Mar 2012 13:54
Last Modified:12 Mar 2012 13:54

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