G-protein activation modulates pseudo-periodic oscillation of Na channel

Majumdar, Sriparna ; Sikdar, Sujit K. (2006) G-protein activation modulates pseudo-periodic oscillation of Na channel Biochemical and Biophysical Research Communications, 347 (2). pp. 444-451. ISSN 0006-291X

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/doi.org/10.1016/j.bbrc.2006.06.104

Abstract

We have shown before that the duration and amplitude of both prolonged (1-160 s) and short (100-1000 ms) depolarizing prepulse altered all the steady-state and kinetic parameters of rNaν1.2a voltage-gated sodium channel in a pseudo-oscillatory fashion with variable time period and amplitude, often superimposed on a linear trend. In this study, we have examined the effect of G-protein activation on pseudo-oscillatory properties of the rNaν1.2a sodium channel α subunit, heterologously expressed in Chinese hamster ovary cells. G-protein modification caused insignificant changes in the slow pseudo-periodic oscillation of the activation properties of sodium channel; only the time period of the oscillation was altered from ∼30 to 21 s. In contrast, G-protein activation abolished the faster component of pseudo-periodic oscillation in steady-state inactivation properties of sodium channel; the conditioning duration dependence of steady-state inactivation becomes monotonic in nature.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Voltage-gated Sodium Channel; G-protein; Periodic Oscillation; Patch-clamp Recording
ID Code:84761
Deposited On:27 Feb 2012 13:54
Last Modified:27 Feb 2012 13:54

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