Kumar, Sunil ; Malhotra, Sarla P. (2008) Partial purification of superoxide dismutase and peroxidase from ber (Zizyphus mauritiana Lamk.) fruit using anion exchange chromatography Physiology and Molecular Biology of Plants, 14 (3). pp. 167-172. ISSN 0971-5894
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Official URL: http://www.springerlink.com/index/L5U74284557U3146...
Related URL: http://dx.doi.org/10.1007/s12298-008-0015-1
Abstract
Partial purification of the two enzymes i.e. superoxide dismutase (SOD) and peroxidase (POX) from ber pulp has been obtained by passing the ammonium sulphate fraction through a diethyl amino ethyl-cellulose (DEAE-Cellulose) column. The fractions showing SOD and POX activity were pooled separately and passed through a Sephadex G100 column for further purification. SOD was purified 12.2 fold with 12.6% yield while POX was purified 15.6 fold with 19.3% yield. Approximate molecular mass for SOD and POX, as judged by gel filtration method was 35.6 and 81.5 kDa, respectively.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Ber Fruit; Superoxide Dismutase; Peroxidase; DEAE-cellulose Chromatography; Fold Purification; Yield; Zizyphus Mauritiana Lamk |
| ID Code: | 84551 |
| Deposited On: | 27 Feb 2012 04:12 |
| Last Modified: | 27 Feb 2012 04:12 |
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