Allantoinase from nodules of pigeonpea (Cajanus cajan)

Amarjit, ; Singh, R. (1985) Allantoinase from nodules of pigeonpea (Cajanus cajan) Phytochemistry, 24 (3). pp. 415-418. ISSN 0031-9422

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Allantoinase was purified about 10-fold from nitrogen fixing root nodules of pigeonpea (Cajanus cajan) using (NH4)2S04 fractionation and chromatography on Sephadex G-100. The purified preparation showed a specific activity of 1.73 nkat/mg protein, Mr of 125 000, pH optimum between 7.5 and 7.7 and Km of 13.3 mM. The enzyme was heat stable up to 70dg and metal ions, except Hg2+, had no effect on the enzyme activity. The enzyme was inhibited significantly by reducing agents. Amino acids, ammonium, nitrate, potential precursors of allantoin and a number of other intermediate metabolites of ureide biosynthetic pathway had no effect on enzyme activity. It is suggested that allantoinase is unlikely to regulate the production of ureides in the nodule tissue.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cajanus cajan; Leguminosae; Pigeonpea; Nodules; Allantoinase; Properties; Intermediate Metabolites; Regulation
ID Code:84519
Deposited On:27 Feb 2012 04:01
Last Modified:27 Feb 2012 04:01

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