Marker-aided genetic divergence analysis in Brassica

Arunachalam, V. ; Verma, Shefali ; Sujata, V. ; Prabhu, K. V. (2005) Marker-aided genetic divergence analysis in Brassica Journal of Genetics, 84 (2). pp. 123-130. ISSN 0022-1333

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Genetic divergence was evaluated in 31 breeding lines from four Brassica species using Mahalanobis' D 2 . A new method of grouping using D 2 values was used to group the 31 lines, based on diagnostic morphological traits (called morphoqts). Isozyme variation of the individual enzymes esterase and glutamate oxaloacetate was quantified by five parameters (called isoqts) developed earlier. Grouping by the same method was also done based on the isoqts, and the grouping by isozymes was compared with that by morphoqts. Overall, there was an agreement of 73% suggesting that isoqts can be used in the choice of parents and also first stage selection of segregants in the laboratory. It was suggested that such an exercise would help to take care of season-bound and field-related problems of breeding. The new isozyme QTs, within lane variance of relative mobility and relative absorption, accounted for about 50% of the total divergence. The utility of the new method and isoqts in cost-effective breeding were highlighted.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Genetic Divergence; Brassica; Isozymes; Mahalanobis’d Grouping
ID Code:841
Deposited On:25 Sep 2010 04:37
Last Modified:16 May 2016 12:02

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