Experimental techniques of two-dimensional correlated spectroscopy

Nagayama, K. ; Anil Kumar, Prof. ; Wuthrich, K. ; Ernst, R. R. (1980) Experimental techniques of two-dimensional correlated spectroscopy Journal of Magnetic Resonance (1969), 40 (2). pp. 321-334. ISSN 0022-2364

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002223...

Related URL: http://dx.doi.org/10.1016/0022-2364(80)90255-3


Two-dimensional (2D) homonuclear correlated spectra manifest connectivities between spin-coupled nuclei and can thus provide assignments of individual spin systems in complex 1H NMR spectra. Two experimental techniques discussed in this paper, spin-echo correlated spectroscopy and foldover-corrected correlated spectroscopy, are particularly suitable versions for handling the large data matrices encountered in work with biological macromolecules. This paper explains the fundamental aspects of these two techniques and the relations with the conventional 2D correlated spectroscopy technique.

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